pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo
(Plasmid #112684)

• Purpose: An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase
• Depositing Lab: Brandon Harvey
• Publication: Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)30062-5. doi: 10.1016/j.neuron.2019.01.035.

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Backbone

• Vector backbone: pOTTC1031
• Backbone manufacturer: NIDA OTTC
• Backbone size w/o insert (bp): 4500
• Total vector size (bp): 6857
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: iRFP713
• Species: Rhodopseudomonas palustris
• GenBank ID: JN247409
• Promoter: CMV-IE
• Tags / Fusion Proteins:
  • Nuclear localization signal (N terminal on insert)
  • 2A-Flpo (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMVf
• 3′ sequencing primer: WPRE_R1

Resource Information

• Supplemental Documents:
  • pOTTC1440 - pAAV CMV-IE Nuc-IRFP-2A-FLPo.ape.ape
• A portion of this plasmid was derived from a plasmid made by: iRFP was obtained from Addgene 31857 FLpo was obtained from Addgene 13793

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry