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Addgene

pQE80L SpyTag-ELP-SpyTag DK
(Plasmid #112635)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112635 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE80L
  • Backbone manufacturer
    Qiagen Inc.
  • Backbone size w/o insert (bp) 4714
  • Total vector size (bp) 5393
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpyTag-ELP-SpyTag DK
  • Species
    Synthetic
  • Insert Size (bp)
    690
  • Mutation
    Contains central cysteine. Reactive aspartic acid in C-terminal SpyTag mutated to lysine (DK).
  • Promoter T5
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CATAAAAAATTTATTTGCTTTGTGAGCGG
  • 3′ sequencing primer CGAGCGTTCTGAACAAATCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE80L SpyTag-ELP-SpyTag DK was a gift from Mark Howarth (Addgene plasmid # 112635 ; http://n2t.net/addgene:112635 ; RRID:Addgene_112635)

  • For your References section:

    Assembling and decorating hyaluronan hydrogels with twin protein superglues to mimic cell-cell interactions. Wieduwild R, Howarth M. Biomaterials. 2018 Oct;180:253-264. doi: 10.1016/j.biomaterials.2018.07.020. Epub 2018 Jul 17. 10.1016/j.biomaterials.2018.07.020 PubMed 30053659
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