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Addgene

pET21-HIS-Tol2
(Plasmid #112486)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112486 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Tol2 Transposase
  • Species
    Synthetic
  • Insert Size (bp)
    2000
  • Entrez Gene
    tpase
  • Promoter T7
  • Tag / Fusion Protein
    • HIS/6x (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21-HIS-Tol2 was a gift from Stephen Ekker (Addgene plasmid # 112486 ; http://n2t.net/addgene:112486 ; RRID:Addgene_112486)

  • For your References section:

    Active recombinant Tol2 transposase for gene transfer and gene discovery applications. Ni J, Wangensteen KJ, Nelsen D, Balciunas D, Skuster KJ, Urban MD, Ekker SC. Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s13100-016-0062-z. eCollection 2016. 62 [pii] PubMed 27042235
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