pGEX Rad
(Plasmid #11227)

• Depositing Lab: Ronald Kahn
• Publication: Zhu et al J Biol Chem. 1995 Mar 3. 270(9):4805-12.

Backbone

• Vector backbone: pGEX-2T
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 4950
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rad
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• Mutation: N39: deletion of amino acids 1-39
• Entrez Gene: RRAD (a.k.a. RAD, RAD1, REM3)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pGEX5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Wildtype Rad residues 40-308: begins at Rad 2nd ATG.