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pCMV_BE4max Citations (28)

Originally described in: Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction.
Koblan LW, Doman JL, Wilson C, Levy JM, Tay T, Newby GA, Maianti JP, Raguram A, Liu DR Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. PubMed Journal

Articles Citing pCMV_BE4max

Articles
Improved base editor for efficient editing in GC contexts in rabbits with an optimized AID-Cas9 fusion. Liu Z, Shan H, Chen S, Chen M, Zhang Q, Lai L, Li Z. FASEB J. 2019 Aug;33(8):9210-9219. doi: 10.1096/fj.201900476RR. Epub 2019 May 9. PubMed
Highly efficient base editing with expanded targeting scope using SpCas9-NG in rabbits. Liu Z, Shan H, Chen S, Chen M, Song Y, Lai L, Li Z. FASEB J. 2020 Jan;34(1):588-596. doi: 10.1096/fj.201901587R. Epub 2019 Nov 26. PubMed
Efficient base editing with high precision in rabbits using YFE-BE4max. Liu Z, Chen S, Shan H, Jia Y, Chen M, Song Y, Lai L, Li Z. Cell Death Dis. 2020 Jan 20;11(1):36. doi: 10.1038/s41419-020-2244-3. PubMed
A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects. Zuo E, Sun Y, Yuan T, He B, Zhou C, Ying W, Liu J, Wei W, Zeng R, Li Y, Yang H. Nat Methods. 2020 May 18. pii: 10.1038/s41592-020-0832-x. doi: 10.1038/s41592-020-0832-x. PubMed

Associated Plasmids
A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing. Grunewald J, Zhou R, Lareau CA, Garcia SP, Iyer S, Miller BR, Langner LM, Hsu JY, Aryee MJ, Joung JK. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y. PubMed

Associated Plasmids
Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Sakata RC, Ishiguro S, Mori H, Tanaka M, Tatsuno K, Ueda H, Yamamoto S, Seki M, Masuyama N, Nishida K, Nishimasu H, Arakawa K, Kondo A, Nureki O, Tomita M, Aburatani H, Yachie N. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0. PubMed

Associated Plasmids
BE4max and AncBE4max Are Efficient in Germline Conversion of C:G to T:A Base Pairs in Zebrafish. Carrington B, Weinstein RN, Sood R. Cells. 2020 Jul 14;9(7). pii: cells9071690. doi: 10.3390/cells9071690. PubMed
CRISPR Start-Loss: A Novel and Practical Alternative for Gene Silencing through Base-Editing-Induced Start Codon Mutations. Chen S, Xie W, Liu Z, Shan H, Chen M, Song Y, Yu H, Lai L, Li Z. Mol Ther Nucleic Acids. 2020 Sep 4;21:1062-1073. doi: 10.1016/j.omtn.2020.07.037. Epub 2020 Jul 31. PubMed
Small-molecule compounds boost genome-editing efficiency of cytosine base editor. Zhao T, Li Q, Zhou C, Lv X, Liu H, Tu T, Tang N, Cheng Y, Liu X, Liu C, Zhao J, Song Z, Wang H, Li J, Gu F. Nucleic Acids Res. 2021 Sep 7;49(15):8974-8986. doi: 10.1093/nar/gkab645. PubMed
Reduced off-target effect of NG-BE4max by using NG-HiFi system. Shan H, Liu Z, Jia Y, Chen S, Chen M, Song Y, Sui T, Lai L, Li Z. Mol Ther Nucleic Acids. 2021 May 19;25:168-172. doi: 10.1016/j.omtn.2021.05.012. eCollection 2021 Sep 3. PubMed
Controllable genome editing with split-engineered base editors. Berrios KN, Evitt NH, DeWeerd RA, Ren D, Luo M, Barka A, Wang T, Bartman CR, Lan Y, Green AM, Shi J, Kohli RM. Nat Chem Biol. 2021 Dec;17(12):1262-1270. doi: 10.1038/s41589-021-00880-w. Epub 2021 Oct 18. PubMed

Associated Plasmids
A general theoretical framework to design base editors with reduced bystander effects. Wang Q, Yang J, Zhong Z, Vanegas JA, Gao X, Kolomeisky AB. Nat Commun. 2021 Nov 11;12(1):6529. doi: 10.1038/s41467-021-26789-5. PubMed
Inhibition of base editors with anti-deaminases derived from viruses. Liu Z, Chen S, Lai L, Li Z. Nat Commun. 2022 Feb 1;13(1):597. doi: 10.1038/s41467-022-28300-0. PubMed
Efficient C-to-G Base Editing with Improved Target Compatibility Using Engineered Deaminase-nCas9 Fusions. Chen S, Liu Z, Lai L, Li Z. CRISPR J. 2022 Jun;5(3):389-396. doi: 10.1089/crispr.2021.0124. Epub 2022 Mar 2. PubMed
Efficient multinucleotide deletions using deaminase-Cas9 fusions in human cells. Chen S, Liu Z, Yu H, Lai L, Li Z. J Genet Genomics. 2022 Oct;49(10):927-933. doi: 10.1016/j.jgg.2022.03.007. Epub 2022 Apr 11. PubMed
Eliminating predictable DNA off-target effects of cytosine base editor by using dual guiders including sgRNA and TALE. Zhou J, Liu Y, Wei Y, Zheng S, Gou S, Chen T, Yang Y, Lan T, Chen M, Liao Y, Zhang Q, Tang C, Liu Y, Wu Y, Peng X, Gao M, Wang J, Zhang K, Lai L, Zou Q. Mol Ther. 2022 Jul 6;30(7):2443-2451. doi: 10.1016/j.ymthe.2022.04.010. Epub 2022 Apr 20. PubMed
Multiplex base- and prime-editing with drive-and-process CRISPR arrays. Yuan Q, Gao X. Nat Commun. 2022 May 19;13(1):2771. doi: 10.1038/s41467-022-30514-1. PubMed

Associated Plasmids
PAM-flexible dual base editor-mediated random mutagenesis and self-activation strategies to improve CRISPRa potency. Lau CH, Huang S, Lam RHW, Tin C. Mol Ther Methods Clin Dev. 2022 May 29;26:26-37. doi: 10.1016/j.omtm.2022.05.005. eCollection 2022 Sep 8. PubMed
Optimization of the base editor BE4max in chicken somatic cells. Xu T, Zhong J, Huang Z, Yu L, Zheng J, Xie L, Sun L, Liu X, Lu Y. Poult Sci. 2022 Dec;101(12):102174. doi: 10.1016/j.psj.2022.102174. Epub 2022 Sep 13. PubMed
Delivery of CRISPR/Cas9 Plasmid DNA by Hyperbranched Polymeric Nanoparticles Enables Efficient Gene Editing. Xiu K, Saunders L, Wen L, Ruan J, Dong R, Song J, Yang D, Zhang J, Xu J, Chen YE, Ma PX. Cells. 2022 Dec 30;12(1):156. doi: 10.3390/cells12010156. PubMed
High-efficiency editing in hematopoietic stem cells and the HUDEP-2 cell line based on in vitro mRNA synthesis. Papaioannou NY, Patsali P, Naiisseh B, Papasavva PL, Koniali L, Kurita R, Nakamura Y, Christou S, Sitarou M, Mussolino C, Cathomen T, Kleanthous M, Lederer CW. Front Genome Ed. 2023 Mar 8;5:1141618. doi: 10.3389/fgeed.2023.1141618. eCollection 2023. PubMed
Base editing strategies to convert CAG to CAA diminish the disease-causing mutation in Huntington's disease. Choi DE, Shin JW, Zeng S, Hong EP, Jang JH, Loupe JM, Wheeler VC, Stutzman HE, Kleinstiver BP, Lee JM. bioRxiv. 2023 Apr 28:2023.04.28.538700. doi: 10.1101/2023.04.28.538700. Preprint. PubMed
An AlphaFold2 map of the 53BP1 pathway identifies a direct SHLD3-RIF1 interaction critical for shieldin activity. Sifri C, Hoeg L, Durocher D, Setiaputra D. EMBO Rep. 2023 Aug 3;24(8):e56834. doi: 10.15252/embr.202356834. Epub 2023 Jun 12. PubMed
APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels. Rieffer AE, Chen Y, Salamango DJ, Moraes SN, Harris RS. CRISPR J. 2023 Oct;6(5):430-446. doi: 10.1089/crispr.2023.0027. Epub 2023 Sep 6. PubMed

Associated Plasmids
Genotoxic effects of base and prime editing in human hematopoietic stem cells. Fiumara M, Ferrari S, Omer-Javed A, Beretta S, Albano L, Canarutto D, Varesi A, Gaddoni C, Brombin C, Cugnata F, Zonari E, Naldini MM, Barcella M, Gentner B, Merelli I, Naldini L. Nat Biotechnol. 2023 Sep 7. doi: 10.1038/s41587-023-01915-4. PubMed
Context base editing for splice correction of IVSI-110 beta-thalassemia. Naiisseh B, Papasavva PL, Papaioannou NY, Tomazou M, Koniali L, Felekis X, Constantinou CG, Sitarou M, Christou S, Kleanthous M, Lederer CW, Patsali P. Mol Ther Nucleic Acids. 2024 Mar 30;35(2):102183. doi: 10.1016/j.omtn.2024.102183. eCollection 2024 Jun 11. PubMed
Sensitive bispecific chimeric T cell receptors for cancer therapy. Simon S, Bugos G, Prins R, Rajan A, Palani A, Heyer K, Stevens A, Zeng L, Thompson K, Price JP, Kluesner MK, Jaeger-Ruckstuhl C, Shabaneh TB, Olson JM, Su X, Riddell SR. Res Sq [Preprint]. 2024 Apr 22:rs.3.rs-4253777. doi: 10.21203/rs.3.rs-4253777/v1. PubMed
Base editing strategies to convert CAG to CAA diminish the disease-causing mutation in Huntington's disease. Choi DE, Shin JW, Zeng S, Hong EP, Jang JH, Loupe JM, Wheeler VC, Stutzman HE, Kleinstiver B, Lee JM. Elife. 2024 Jun 13;12:RP89782. doi: 10.7554/eLife.89782. PubMed

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