pAAV-hSynapsin1-FLEx-axon-GCaMP6s-P2A-mRuby3
(Plasmid #112008)

• Purpose: For cre-dependent enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3
• Depositing Lab: Lin Tian
• Publication: Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280. doi: 10.1038/s41593-018-0211-4. Epub 2018 Aug 20.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 5109
• Total vector size (bp): 7302
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: axon-GCaMP6s-P2A-mRuby3
• Alt name: GAP43-GCaMP6s-P2A-mRuby3
• Species: Synthetic
• Insert Size (bp): 2193
• GenBank ID: MH282424
• Promoter: hSynapsin1
• Tag / Fusion Protein:
  • GAP43 palmitoylation domain (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: GAAGGCGCGCTGACGTCACTCGCCG
• 3′ sequencing primer: CAAATTTTGTAATCCAGAGGTTGATT

Resource Information

• Supplemental Documents:
  • pAAV-hSynapsin1-FLEX-axon-G6s-P2A-mRuby3.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry