pBacMam2-DiEx-LIC-C entry vector
(Plasmid #111752)

• Purpose: (Empty Backbone) Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with C-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells
• Depositing Lab: Cheryl Arrowsmith
• Publication: Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 10.1074/jbc.RA118.007204. Epub 2019 Mar 6.

Backbone

• Vector backbone: pBacMam2-DiEx-LIC
• Backbone size (bp): 15911
• Vector type: Mammalian Expression ; Baculovirus Expression
• Tag / Fusion Protein:
  • FLAG (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • FLAG (C terminal on backbone)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains most of the HTT gene: amino acids (AA) 1-6, a cloning site, and AA 131-3144. It lacks the polyQ region so that other polyQ lengths can be introduced. Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.