pAC37
(Plasmid
#111618)
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PurposeFluorescent biosensor for gauging the activity of the PQS quorum sensing system in Pseudomonas aeruginosa
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 111618 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUCP22Not
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Vector typeBacterial Expression
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Selectable markersAmpicillin
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePpqsA-gfp(ASV)
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GFP(AAV)- GFP variant with a shorter half life.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAC37 was a gift from Tim Tolker-Nielsen (Addgene plasmid # 111618 ; http://n2t.net/addgene:111618 ; RRID:Addgene_111618) -
For your References section:
Effects of iron on DNA release and biofilm development by Pseudomonas aeruginosa. Yang L, Barken KB, Skindersoe ME, Christensen AB, Givskov M, Tolker-Nielsen T. Microbiology. 2007 May;153(Pt 5):1318-28. doi: 10.1099/mic.0.2006/004911-0. 10.1099/mic.0.2006/004911-0 PubMed 17464046