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pET-SUMO-hGSDMD
(Plasmid #111559)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111559 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET SUMO
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 5600
  • Total vector size (bp) 7098
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Gasdermin D
  • Alt name
    GSDMD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1455
  • Entrez Gene
    GSDMD (a.k.a. DF5L, DFNA5L, FKSG10, GSDMDC1)
  • Promoter T7
  • Tag / Fusion Protein
    • His, SUMO (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-SUMO-hGSDMD was a gift from Hongbo Luo (Addgene plasmid # 111559 ; http://n2t.net/addgene:111559 ; RRID:Addgene_111559)

  • For your References section:

    Gasdermin D Exerts Anti-inflammatory Effects by Promoting Neutrophil Death. Kambara H, Liu F, Zhang X, Liu P, Bajrami B, Teng Y, Zhao L, Zhou S, Yu H, Zhou W, Silberstein LE, Cheng T, Han M, Xu Y, Luo HR. Cell Rep. 2018 Mar 13;22(11):2924-2936. doi: 10.1016/j.celrep.2018.02.067. 10.1016/j.celrep.2018.02.067 PubMed 29539421
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