IKK-2 S177E S181E
(Plasmid #11105)

• Purpose: Mammalian expression of constitutively active human IKK-2
• Depositing Lab: Anjana Rao
• Publication: Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

Backbone

• Vector backbone: pCMV2
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IKK-2
• Species: H. sapiens (human)
• Insert Size (bp): 2300
• Mutation: S177E and S181E, constitutively active
• Entrez Gene: IKBKB (a.k.a. IKK-2, IKK-beta, IKK2, IKKB, IMD15, IMD15A, IMD15B, NFKBIKB)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 32 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry