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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11100 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneGFP-RV-DV
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Backbone manufacturerGenetics Institute
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNFAT1
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SpeciesM. musculus (mouse)
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Insert Size (bp)2800
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Entrez GeneNfatc2 (a.k.a. AI607462, NF-ATc2, NF-ATp, NFAT1, NFAT1-D, Nfatp)
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Tags
/ Fusion Proteins
- 3xHA (N terminal on insert)
- GFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer IRES-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
WT NFAT1 was a gift from Anjana Rao (Addgene plasmid # 11100 ; http://n2t.net/addgene:11100 ; RRID:Addgene_11100) -
For your References section:
NFAT1 and NFAT2 are positive regulators of IL-4 gene transcription. Monticelli S, Rao A. Eur J Immunol. 2002 Oct . 32(10):2971-8. 10.1002/1521-4141(2002010)32:10<2971::AID-IMMU2971>3.0.CO;2-G PubMed 12355451
