pET15TV-L-YpODH
(Plasmid #110806)

• Purpose: Expresses YpODH and opine dehydrogenase from Yersinia pests
• Depositing Lab: Audrey Lamb
• Publication: McFarlane et al J Biol Chem. 2018 Apr 4. pii: RA118.002007. doi: 10.1074/jbc.RA118.002007.

Backbone

• Vector backbone: pET15TV-L
• Backbone manufacturer: Cheryl Arrowsmith
• Backbone size w/o insert (bp): 7746
• Total vector size (bp): 9126
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YPO1347
• Alt name: YpODH - opine dehydrogenase from Yersinia pests
• Insert Size (bp): 1380
• Entrez Gene: YPO1347 (a.k.a. YPO1347)
• Promoter: T7
• Tag / Fusion Protein:
  • Hexahistidine tag (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pET Upstream
• 3′ sequencing primer: T7 Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene was synthesized and cloned by Genscript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry