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Addgene

pET15TV-L-SaODH
(Plasmid #110804)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110804 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET15TV-L
  • Backbone manufacturer
    Cheryl Arrowsmith
  • Backbone size w/o insert (bp) 7746
  • Total vector size (bp) 9057
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SAV2468
  • Alt name
    SaODH - opine dehydrogenase from Staphylococcus aureus
  • Insert Size (bp)
    1311
  • Entrez Gene
    SAV2468 (a.k.a. SAV2468)
  • Promoter T7
  • Tag / Fusion Protein
    • Hexahistidine tag (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15TV-L-SaODH was a gift from Audrey Lamb (Addgene plasmid # 110804 ; http://n2t.net/addgene:110804 ; RRID:Addgene_110804)
  • For your References section:

    Biosynthesis of an Opine Metallophore by Pseudomonas aeruginosa. McFarlane JS, Lamb AL. Biochemistry. 2017 Nov 14;56(45):5967-5971. doi: 10.1021/acs.biochem.7b00804. Epub 2017 Nov 3. 10.1021/acs.biochem.7b00804 PubMed 29091735