pET15TV-L-SaODH
(Plasmid
#110804)
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PurposeExpresses SaODH and opine dehydrogenase from Staphylococcus aureus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 110804 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET15TV-L
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Backbone manufacturerCheryl Arrowsmith
- Backbone size w/o insert (bp) 7746
- Total vector size (bp) 9057
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSAV2468
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Alt nameSaODH - opine dehydrogenase from Staphylococcus aureus
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Insert Size (bp)1311
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Entrez GeneSAV2468 (a.k.a. SAV2468)
- Promoter T7
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Tag
/ Fusion Protein
- Hexahistidine tag (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pET Upstream
- 3′ sequencing primer T7 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET15TV-L-SaODH was a gift from Audrey Lamb (Addgene plasmid # 110804 ; http://n2t.net/addgene:110804 ; RRID:Addgene_110804) -
For your References section:
Biosynthesis of an Opine Metallophore by Pseudomonas aeruginosa. McFarlane JS, Lamb AL. Biochemistry. 2017 Nov 14;56(45):5967-5971. doi: 10.1021/acs.biochem.7b00804. Epub 2017 Nov 3. 10.1021/acs.biochem.7b00804 PubMed 29091735