pET15TV-L-SaNAS
(Plasmid #110803)

• Purpose: Expresses SaNAS a nicotianamine synthase from Staphylcoccus aureus
• Depositing Lab: Audrey Lamb
• Publication: McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-5971. doi: 10.1021/acs.biochem.7b00804. Epub 2017 Nov 3.

Backbone

• Vector backbone: pET15TV-L
• Backbone manufacturer: Cheryl Arrowsmith
• Backbone size w/o insert (bp): 7746
• Total vector size (bp): 8574
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SAV2469
• Alt name: SaNAS - nicotianamine synthase from Staphylococcus aureus
• Insert Size (bp): 828
• Entrez Gene: SAV2469 (a.k.a. SAV2469)
• Promoter: T7
• Tag / Fusion Protein:
  • Hexahistidine tag (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pET Upstream
• 3′ sequencing primer: T7 Reverse

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Gene was synthesized and cloned into pET15TV-L by Genscript.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry