pSPL
(Plasmid
#110743)
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Purpose(Empty Backbone) Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110743 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSPL
- Backbone size (bp) 2857
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Vector typeInsect Expression
- Promoter Polyhedrin and p10 promoters
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Selectable markersSpectinomycin
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSPL was a gift from Tim Richmond (Addgene plasmid # 110743 ; http://n2t.net/addgene:110743 ; RRID:Addgene_110743) -
For your References section:
Protein complex expression by using multigene baculoviral vectors. Fitzgerald DJ, Berger P, Schaffitzel C, Yamada K, Richmond TJ, Berger I. Nat Methods. 2006 Dec;3(12):1021-32. doi: 10.1038/nmeth983. 10.1038/nmeth983 PubMed 17117155
Map uploaded by the depositor.
