HMEKc-srcM
(Plasmid #1107)

• Depositing Lab: Susan Lindquist
• Publication: Xu et al Proc Natl Acad Sci U S A 1999 Jan 5;96(1):109-14.

Backbone

• Vector backbone: pRS313
• Backbone manufacturer: ATCC
• Backbone size w/o insert (bp): 4967
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: c-src
• Alt name: tyrosine kinase
• Alt name: c-src K295M
• Alt name: CSK
• Species: Rous sarcoma virus
• Mutation: MET3 promoter inserted in EcoRI and BamHI of pRS313 (HMEK, unpublished) K295M mutation of c-src

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: M13R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry