p175 Grg-1s HA
(Plasmid
#11064)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11064 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMH
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Backbone manufacturerRoche Molecular Biochemicals
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGrg1-S
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Alt nameGroucho
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Alt nameGrg1 short
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SpeciesM. musculus (mouse)
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Insert Size (bp)600
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Entrez GeneTle1 (a.k.a. C230057C06Rik, Estm14, Grg-1, Grg1, Tle4l)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p175 Grg-1s HA was a gift from Ramesh Shivdasani (Addgene plasmid # 11064 ; http://n2t.net/addgene:11064 ; RRID:Addgene_11064) -
For your References section:
Characterization of a novel mammalian Groucho isoform and its role in transcriptional regulation. Lepourcelet M, Shivdasani RA. J Biol Chem. 2002 Dec 6. 277(49):47732-40. 10.1074/jbc.M208154200 PubMed 12359720
