ELONGIN BC (XX01TCEB1A-c001)
(Plasmid #110274)

• Purpose: Elongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin C
• Depositing Lab: Nicola Burgess-Brown
• Publication: SGC Oxford April 2018 plasmids (unpublished)

Backbone

• Vector backbone: pACYC-Duet1
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Elongin BC
• Alt name: co-expresses Elongin B and Elongin C
• Species: H. sapiens (human)
• Mutation: Elongin B is full length. Elongin C contains a.a. 17-112.
• Entrez Gene: ELOC (a.k.a. SIII, TCEB1)
• Entrez Gene: ELOB (a.k.a. SIII, TCEB2)
• Promoter: T7

Cloning Information

• Cloning method: Unknown
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: pDuetDOWN-1 (5'-TTGTACACGGCCGCATAATC-3') or pDuetUP2 (5'-GATTATGCGGCCGTGTACAA-3')
• 3′ sequencing primer: T7-term

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full length Elongin B was cloned into MCS1 NcoI site of pACYC-Duet1.

Elongin C (a.a. 17-112) was cloned in 2nd MCS with stop codon before any 3’ vector S tag. Therefore, these inserts produce untagged proteins.

Note: ATG from NdeI site precedes internal start Met17 of Elongin C giving start sequence MMYVK...

2C9W: https://www.thesgc.org/structures/2C9W.