pTK-enhNC1.1_M3-MCS-2A-eGFP-SV40pA
(Plasmid
#110203)
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PurposeRecipient vector for Cranial Neural Crest-specific expression of protein of interest
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110203 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTK
- Backbone size w/o insert (bp) 4979
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameenhNC1.1_M3-TF_MCS-2A-eGFP-SV40pA
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SpeciesG. gallus (chicken)
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Tag
/ Fusion Protein
- eGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains EcoRV restriction enzyme site for cloning protein of interest.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTK-enhNC1.1_M3-MCS-2A-eGFP-SV40pA was a gift from Tatjana Sauka-Spengler (Addgene plasmid # 110203 ; http://n2t.net/addgene:110203 ; RRID:Addgene_110203)
