EGFR (del3) L747-E749del, A750P
(Plasmid #11015)

• Purpose: Retroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cells
• Depositing Lab: Matthew Meyerson
• Publication: Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Backbone

• Vector backbone: pBABE puro
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFR (del3)
• Alt name: epidermal growth factor receptor
• Alt name: ERBB
• Species: H. sapiens (human)
• Insert Size (bp): 3630
• Mutation: L747-E749 deleted, A750P
• Entrez Gene: EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SnaBI (destroyed during cloning)
• 3′ cloning site: SnaBI (destroyed during cloning)
• 5′ sequencing primer: pBABE5'

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Author has deposited the sequence of the insert to Addgene. Click on "sequence" to view. Mutation made by using the following primers to PCR from plasmid 11011: 5'-GTCGCTATCAAGGAACCAACATCTCCGAAA-3' and 5'-TTTCGGAGATGTTGGTTCCTTGATAGCGAC-3'