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Addgene

Flag-Axin1
(Plasmid #109370)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109370 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-tag2b
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4324
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Axin1
  • Species
    H. sapiens (human)
  • Entrez Gene
    AXIN1 (a.k.a. AXIN, PPP1R49)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Axin1 sequence was derived from PMID 12192039.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-Axin1 was a gift from Mariann Bienz (Addgene plasmid # 109370 ; http://n2t.net/addgene:109370 ; RRID:Addgene_109370)

  • For your References section:

    Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating beta-catenin. Fiedler M, Mendoza-Topaz C, Rutherford TJ, Mieszczanek J, Bienz M. Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):1937-42. doi: 10.1073/pnas.1017063108. Epub 2011 Jan 18. 10.1073/pnas.1017063108 PubMed 21245303
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