pDB026g
(Plasmid #109263)

• Purpose: Accessory plasmid for PACE of MjTyrRS variants
• Depositing Lab: David Liu
• Publication: Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038/nchembio.2474.

Backbone

• Vector backbone: SC101
• Backbone manufacturer: David Liu Lab
• Total vector size (bp): 7198
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Ppsp_pIII(P29*, P83*, T177*, Y184*); luxAB; PproK_TyrT(Opt, CUA);
• Species: Synthetic
• Mutation: amber codon at position 29, 83, 177, and 184 of gIII
• Promoter: Ppsp and PproK

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • pDB026g.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry