pET41-MBP-t-A3A-t-His
(Plasmid #109231)

• Purpose: Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease
• Depositing Lab: Rahul Kohli
• Publication: Schutsky et al Nat Biotechnol. 2018 Oct 8. pii: nbt.4204. doi: 10.1038/nbt.4204.

Backbone

• Vector backbone: pET41
• Total vector size (bp): 6837
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: human APOBEC3A
• Alt name: A3A
• Species: H. sapiens (human); Optimized for E. coli expression
• Insert Size (bp): 597
• Entrez Gene: APOBEC3A (a.k.a. A3A, ARP3, PHRBN, bK150C2.1)
• Tags / Fusion Proteins:
  • MBP (N terminal on insert)
  • His (C terminal on insert)

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry