pIDS-RESTmut full-length
(Plasmid
#109162)
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PurposeEncodes human full-length REST/NRSF C402S to be expressed in a baculovirus/insect cell expression system
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109162 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIDS
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Backbone manufacturerGeneva Biotech
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehuman full-length REST/NRSF
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SpeciesH. sapiens (human)
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Insert Size (bp)3294
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MutationCys402Ser
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Entrez GeneREST (a.k.a. DFNA27, GINGF5, HGF5, NRSF, WT6, XBR)
- Promoter p10
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer p10
- 3′ sequencing primer HSV_rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIDS-RESTmut full-length was a gift from Monika Golas (Addgene plasmid # 109162 ; http://n2t.net/addgene:109162 ; RRID:Addgene_109162) -
For your References section:
Stepwise assembly of functional C-terminal REST/NRSF transcriptional repressor complexes as a drug target. Inui K, Zhao Z, Yuan J, Jayaprakash S, Le LTM, Drakulic S, Sander B, Golas MM. Protein Sci. 2017 May;26(5):997-1011. doi: 10.1002/pro.3142. Epub 2017 Mar 12. 10.1002/pro.3142 PubMed 28218430
