pDS-Red-N1 GABAA beta3
(Plasmid
#109143)
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PurposeThe expression vector carries the red fluorescent protein tagged at the C-terminus of beta3 subunit of the GABAa receptor.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109143 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDS-Red1-N1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGABAa receptor
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Mutationbeta3 subunit of the GABAa receptor
- Promoter CMV
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Tag
/ Fusion Protein
- RFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDS-Red-N1 GABAA beta3 was a gift from Stefano Vicini (Addgene plasmid # 109143 ; http://n2t.net/addgene:109143 ; RRID:Addgene_109143)
