NK641
(Plasmid
#109100)
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PurposeReporter gene expression in S. rosetta
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 109100 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDSB_0093
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Vector typeChoanoflagellate Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePTSG_07215
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Alt namepEFL_PTSG_07215_mTFP1_3'act
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pDSB_0101 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NK641 was a gift from Nicole King (Addgene plasmid # 109100 ; http://n2t.net/addgene:109100 ; RRID:Addgene_109100) -
For your References section:
Transfection of choanoflagellates illuminates their cell biology and the ancestry of animal septins. Booth DS, Szmidt-Middleton H, King N. Mol Biol Cell. 2018 Dec 1;29(25):3026-3038. doi: 10.1091/mbc.E18-08-0514. Epub 2018 Oct 3. 10.1091/mbc.E18-08-0514 PubMed 30281390