pYPQ141-ZmUbi-RZ-Fn
(Plasmid
#108864)
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Purpose(Empty Backbone) FnCpf1 Gateway crRNA entry plasmid using Zea mays Ubi promoter and double ribozyme processing
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108864 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYPQ141-ZmUbi-RZ-Fn
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Vector typePlant Expression, CRISPR ; Gateway compatible crRNA entry clone
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYPQ141-ZmUbi-RZ-Fn was a gift from Yiping Qi (Addgene plasmid # 108864 ; http://n2t.net/addgene:108864 ; RRID:Addgene_108864) -
For your References section:
Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites. Zhong Z, Zhang Y, You Q, Tang X, Ren Q, Liu S, Yang L, Wang Y, Liu X, Liu B, Zhang T, Zheng X, Le Y, Zhang Y, Qi Y. Mol Plant. 2018 Jul 2;11(7):999-1002. doi: 10.1016/j.molp.2018.03.008. Epub 2018 Mar 20. 10.1016/j.molp.2018.03.008 PubMed 29567452