pCR3-vsv-CenpB 1-166-Sgo1 N61I
(Plasmid
#108498)
-
Purposeexpression of vsv-CenpB 1-166-Sgo1 N61I (no PP2A binding)
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108498 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCR3
- Backbone size w/o insert (bp) 5000
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCenpB-Shugoshin 1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2100
-
MutationCenpB 1-166 fusion before Sgo1, no PP2A binding N61I
-
Entrez GeneSGO1 (a.k.a. CAID, NY-BR-85, SGO, SGOL1)
- Promoter CMV
-
Tag
/ Fusion Protein
- VSV (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (unknown if destroyed)
- 3′ cloning site Pst1 (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCR3-vsv-CenpB 1-166-Sgo1 N61I was a gift from Susanne Lens (Addgene plasmid # 108498 ; http://n2t.net/addgene:108498 ; RRID:Addgene_108498) -
For your References section:
Shugoshin-1 balances Aurora B kinase activity via PP2A to promote chromosome bi-orientation. Meppelink A, Kabeche L, Vromans MJ, Compton DA, Lens SM. Cell Rep. 2015 Apr 28;11(4):508-15. doi: 10.1016/j.celrep.2015.03.052. Epub 2015 Apr 16. 10.1016/j.celrep.2015.03.052 PubMed 25892238
