Skip to main content
Addgene

vsv-Sgo1 N61I-GFP
(Plasmid #108497)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108497 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Shugoshin 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1584
  • Mutation
    no PP2A binding N61I
  • Entrez Gene
    SGO1 (a.k.a. CAID, NY-BR-85, SGO, SGOL1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • VSV (N terminal on insert)
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HinDIII (unknown if destroyed)
  • 3′ cloning site HinDIII (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    vsv-Sgo1 N61I-GFP was a gift from Susanne Lens (Addgene plasmid # 108497 ; http://n2t.net/addgene:108497 ; RRID:Addgene_108497)

  • For your References section:

    Shugoshin-1 balances Aurora B kinase activity via PP2A to promote chromosome bi-orientation. Meppelink A, Kabeche L, Vromans MJ, Compton DA, Lens SM. Cell Rep. 2015 Apr 28;11(4):508-15. doi: 10.1016/j.celrep.2015.03.052. Epub 2015 Apr 16. 10.1016/j.celrep.2015.03.052 PubMed 25892238
Related items:
Commonly requested with: