vsv-Sgo1-GFP
(Plasmid
#108494)
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Purposeexpression of vsv-Sgo1-EGFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108494 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameShugoshin 1
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SpeciesH. sapiens (human)
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Insert Size (bp)1584
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Entrez GeneSGO1 (a.k.a. CAID, NY-BR-85, SGO, SGOL1)
- Promoter CMV
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Tags
/ Fusion Proteins
- VSV (N terminal on insert)
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HinDIII (unknown if destroyed)
- 3′ cloning site HinDIII (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
vsv-Sgo1-GFP was a gift from Susanne Lens (Addgene plasmid # 108494 ; http://n2t.net/addgene:108494 ; RRID:Addgene_108494) -
For your References section:
Shugoshin-1 balances Aurora B kinase activity via PP2A to promote chromosome bi-orientation. Meppelink A, Kabeche L, Vromans MJ, Compton DA, Lens SM. Cell Rep. 2015 Apr 28;11(4):508-15. doi: 10.1016/j.celrep.2015.03.052. Epub 2015 Apr 16. 10.1016/j.celrep.2015.03.052 PubMed 25892238
