xCas9 3.7
(Plasmid #108379)

• Purpose: Mammalian expression vector for xCas9 3.7
• Depositing Lab: David Liu
• Publication: Hu et al Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.

Backbone

• Vector backbone: pCMV
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: xCas9 3.7
• Species: Synthetic
• Insert Size (bp): 4140
• Mutation: A262T, R324L, S409I, E480K, E543D, M694I, E1219V
• Tag / Fusion Protein:
  • NLS (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV Forward

Resource Information

• Supplemental Documents:
  • xCas9 3.7.gb
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry