pRVV669
(Plasmid
#108283)
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PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108283 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript SK+
- Total vector size (bp) 10212
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePhiC31 attP sites
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SpeciesStreptomyces phage PhiC31
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRVV669 was a gift from Richard Mann (Addgene plasmid # 108283 ; http://n2t.net/addgene:108283 ; RRID:Addgene_108283) -
For your References section:
Robust PhiC31-Mediated Genome Engineering in Drosophila melanogaster Using Minimal attP/attB Phage Sites. Voutev R, Mann RS. G3 (Bethesda). 2018 Mar 9. pii: g3.118.200051. doi: 10.1534/g3.118.200051. 10.1534/g3.118.200051 PubMed 29523637
Map uploaded by the depositor.
