Skip to main content
Addgene

pGreen2x35SLUC
(Plasmid #108228)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108228 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGreenII0800-LUC
  • Backbone manufacturer
    Hellens et al. Plant Methods 2005, 1:13 doi:10.1186/1746-4811-1-13
  • Modifications to backbone
    added a promoter (2x35S) so that the original plasmid used for transcriptional fusions, is now suitable for translational fusions
  • Vector type
    Plant Expression, Luciferase
  • Promoter 2x35S
  • Tag / Fusion Protein
    • Firefly Luciferase (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGreen2x35SLUC was a gift from Alejandro Ferrando (Addgene plasmid # 108228 ; http://n2t.net/addgene:108228 ; RRID:Addgene_108228)

  • For your References section:

    Quantification of Xylem-Specific Thermospermine-Dependent Translation of SACL Transcripts with Dual Luciferase Reporter System. Solé-Gil A, Úrbez C, Ferrando A, Blázquez MA. Methods Mol Biol. 2024;2722:79-87. doi: 10.1007/978-1-0716-3477-6_6. 10.1007/978-1-0716-3477-6_6 PubMed 37897601
Related items:
Commonly requested with: