pUCdeltaSS-ERE
(Plasmid
#108217)
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PurposeYeast reporter gene with estrogen response element (ERE) upstream of CYC1 promoter driving lacZ expression
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108217 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC18
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameERE-CYC1-lacZ
- Promoter CYC1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ERE sequence is 5-tcgagtcaggtcacagtgacctgatcaaag
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUCdeltaSS-ERE was a gift from Didier Picard & Keith Yamamoto (Addgene plasmid # 108217 ; http://n2t.net/addgene:108217 ; RRID:Addgene_108217) -
For your References section:
Reduced levels of hsp90 compromise steroid receptor action in vivo. Picard D, Khursheed B, Garabedian MJ, Fortin MG, Lindquist S, Yamamoto KR. Nature 1990 Nov 8;348(6297):166-8. 10.1038/348166a0 PubMed 2234079