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Addgene

PKCalpha.K/N.FLAG
(Plasmid #10806)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10806 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKC alpha
  • Alt name
    PKC-A
  • Alt name
    PRKCA
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    2300
  • Mutation
    Kinase dead mutant: K368N (Lys 368 to Asn)
  • GenBank ID
    NM_174435
  • Entrez Gene
    PRKCA (a.k.a. PKRCA)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PKCalpha.K/N.FLAG was a gift from Alex Toker (Addgene plasmid # 10806 ; http://n2t.net/addgene:10806 ; RRID:Addgene_10806)

  • For your References section:

    Effect of overexpression of protein kinase C alpha on rat lacrimal gland protein secretion. Hodges RR, Kazlauskas A, Toker A, Dartt DA. Adv Exp Med Biol. 2002 . 506(Pt A):237-41. PubMed 12613914
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