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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 10797 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV6
- Backbone size w/o insert (bp) 4650
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePKC epsilon
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SpeciesH. sapiens (human)
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Insert Size (bp)2300
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Entrez GenePRKCE (a.k.a. PKCE, nPKC-epsilon)
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Tags
/ Fusion Proteins
- Myr (N terminal on insert)
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII or KpnI? (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Constitutively active. Myr sequence from p60src.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Myr.PKCepsilon.FLAG was a gift from Alex Toker (Addgene plasmid # 10797 ; http://n2t.net/addgene:10797 ; RRID:Addgene_10797) -
For your References section:
Regulation of novel protein kinase C epsilon by phosphorylation. Cenni V, Doppler H, Sonnenburg ED, Maraldi N, Newton AC, Toker A. Biochem J. 2002 May 1. 363(Pt 3):537-45. 10.1042/0264-6021:3630537 PubMed 11964154