pDawn-Ag43
(Plasmid #107742)

• Purpose: Expresses Ag43 under the control of a blue-light transcriptional regulator
• Depositing Lab: Ingmar Riedel-Kruse
• Publication: Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):3698-3703. doi: 10.1073/pnas.1720676115. Epub 2018 Mar 19.

Backbone

• Vector backbone: pDawn
• Backbone manufacturer: Andreas Moeglich Lab
• Backbone size w/o insert (bp): 7293
• Total vector size (bp): 10440
• Modifications to backbone: replaced KanR with specR
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Ag43
• Alt name: flu
• Insert Size (bp): 3147
• Mutation: removed all pstI cut sites with silent mutations, added RBS
• Entrez Gene: flu (a.k.a. b2000, ECK1993, agn, agn43, yeeQ, yzzX)
• Promoter: pLambda

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: ctctggcggtgataatggttgc
• 3′ sequencing primer: GAGCCCCCGATTTAGAGC

Resource Information

• Supplemental Documents:
  • pDawn-Ag43.gb
• A portion of this plasmid was derived from a plasmid made by: iGEM BioBricks BBa_K346007
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Ag43 contains a G981S variant compared to the NCBI reference (WP_000820410.1); plasmid functions as described in the associated publication.