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Purpose(Empty Backbone) Control expression plasmid for 3xFLAG-tagged pN3-contructs
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107717 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepN3
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Backbone manufacturerGuntram Suske (Addgene plasmid # 24544)
- Backbone size (bp) 3996
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Vector typeMammalian Expression
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Tag
/ Fusion Protein
- 3xFlag (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- 3xFlag (N terminal on backbone)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pN3-3xFlag is a derivative of the pN3-Control vector (Addgene 24544)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pN3-3xFlag-Control was a gift from Guntram Suske (Addgene plasmid # 107717 ; http://n2t.net/addgene:107717 ; RRID:Addgene_107717) -
For your References section:
MGA, L3MBTL2 and E2F6 determine genomic binding of the non-canonical Polycomb repressive complex PRC1.6. Stielow B, Finkernagel F, Stiewe T, Nist A, Suske G. PLoS Genet. 2018 Jan 30;14(1):e1007193. doi: 10.1371/journal.pgen.1007193. 10.1371/journal.pgen.1007193 PubMed 29381691
