p3E-U6a-U6c
(Plasmid #107599)

• Purpose: An entry vector with U6a and U6c promoter driving guide RNAs expression
• Depositing Lab: Qing Deng
• Publication: Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii: dmm.033027. doi: 10.1242/dmm.033027.

Backbone

• Vector backbone: p3E
• Backbone size w/o insert (bp): 2651
• Total vector size (bp): 4088
• Modifications to backbone: a polyA was inserted into the backbone
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: U6a promoter
• Species: D. rerio (zebrafish)
• Insert Size (bp): 550

Gene/Insert 2

• Gene/Insert name: U6c promoter
• Insert Size (bp): 604

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

infusion cloning was used