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pLenti CMV Puro DEST (w118-1) SDC3
(Plasmid #107501)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107501 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti CMV Puro DEST (w118-1)
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SDC3 (codon optimized)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1329
  • Mutation
    Codon optimized sequence
  • Entrez Gene
    SDC3 (a.k.a. SDCN, SYND3)
  • Promoter CMV

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CMV
  • 3′ sequencing primer Dest Rev (GGAGCAACATAGTTA)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti CMV Puro DEST (w118-1) SDC3 was a gift from Alejandro Gutierrez (Addgene plasmid # 107501 ; http://n2t.net/addgene:107501 ; RRID:Addgene_107501)

  • For your References section:

    PRC2 loss induces chemoresistance by repressing apoptosis in T cell acute lymphoblastic leukemia. Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB, Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, Gutierrez A. J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570. 10.1084/jem.20180570 PubMed 30404791
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