pDisplay-Rncp-iGluSnFR1
(Plasmid #107336)

• Purpose: Red fluorescent glutamate sensor with ncp topology. Membrane-displayed
• Depositing Lab: Robert Campbell
• Publication: Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021/acschembio.7b01085.

Backbone

• Vector backbone: pDisplay
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5268
• Total vector size (bp): 6909
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rncp-iGluSnFR1
• Alt name: mApple based and noncircularly permutated glutamate sensor
• Species: Synthetic
• Insert Size (bp): 1641
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: PstI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • pDisplay-Rncp-iGluSnFR1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that mutation M178K in the Neomycin resistance cassette was found during Addgene's quality control. Neomycin selection has not been tested for this plasmid, but should not affect sensor function.