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Purpose(Empty Backbone) single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
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| Plasmid | 106477 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCROPseq-Guide-EFS-SpCas9-P2A-EGFP
- Backbone size (bp) 12951
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Modifications to backbonereplaced EGFP with Blasticidin resistance gene
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Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter EFS
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byBlasticidin resistance gene from Feng Zhang lab's lentiCas9-Blast plasmid (Addgene#52962); backbone originally derived from the CROPseq-Guide construct from Christopher Bock's Lab (Addgene#86708)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CROPseq-EFS-SpCas9-P2A-blast was a gift from Alex Hewitt (Addgene plasmid # 106477 ; http://n2t.net/addgene:106477 ; RRID:Addgene_106477)
