pET28a_T7-ARG1
(Plasmid #106473)

• Purpose: Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli
• Depositing Lab: Mikhail Shapiro
• Publication: Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi: 10.1038/nature25021.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5374
• Total vector size (bp): 12129
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ARG1
• Alt name: Acoustic Reporter Gene 1
• Alt name: Hybrid Gas Vesicle gene cluster
• Species: Anabaena flos-aquae and Bacillus megaterium
• Insert Size (bp): 6755
• Promoter: T7

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7_Forward
• 3′ sequencing primer: T7 terminator_Reverse

Resource Information

• Supplemental Documents:
  • pET28a_T7-ARG1.gb
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the Addgene verified sequence differs slightly from the depositor's reference sequence linked in the Supplemental Documents section. These differences do not affect plasmid function.