pNP160
(Plasmid
#106362)
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PurposeTo insert a single copy of a construct at a site on Chr II, corresponding to RDE-1 expressed specifically in the intestine, for tissue-specific RNAi.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 106362 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNP154
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namettTi5605_SEC_mtl-2p_RDE-1_3'rde-1
Cloning Information
- Cloning method Unknown
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid contains ttTi5605_SEC_mtl-2p_RDE-1_3'rde-1. Insertion and selection = Chr II ttTi5605 +SEC
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNP160 was a gift from Jonathan Ewbank (Addgene plasmid # 106362 ; http://n2t.net/addgene:106362 ; RRID:Addgene_106362)