pNP151
(Plasmid
#106359)
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PurposeTo insert a single copy of a construct at a site on Chr II, corresponding to a mKate-labelled LifeAct expressed specifically in the adult epidermis.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 106359 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCFJ151
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecol-62p_lifeact_mkate2_3'nmy-2
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid contains col-62p_lifeact_mkate2_3'nmy-2. Insertion and selection = Chr II ttTi5605 +unc-119
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNP151 was a gift from Jonathan Ewbank (Addgene plasmid # 106359 ; http://n2t.net/addgene:106359 ; RRID:Addgene_106359) -
For your References section:
Microtubule plus-end dynamics link wound repair to the innate immune response. Taffoni C, Omi S, Huber C, Mailfert S, Fallet M, Rupprecht JF, Ewbank JJ, Pujol N. Elife. 2020 Jan 29;9. pii: 45047. doi: 10.7554/eLife.45047. 10.7554/eLife.45047 PubMed 31995031
