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Addgene

sgLenti-orthogonal
(Plasmid #105997)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105997 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR ; S.pyogenes sgRNA expression vector
  • Promoter U6
  • Selectable markers
    Puromycin ; mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Dual sgRNA vector to express one sgRNA for S.pyogenes Cas9 and one sgRNA for S.aureus Cas9. Cloning is done in two steps: 1.) AarI digest to clone the S.pyogenes sgRNA and after preparation of the successfully cloned plasmid 2.) BfuAI digest followed by cloning of the S.aureus sgRNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sgLenti-orthogonal was a gift from Mike McManus (Addgene plasmid # 105997 ; http://n2t.net/addgene:105997 ; RRID:Addgene_105997)

  • For your References section:

    Dual gene activation and knockout screen reveals directional dependencies in genetic networks. Boettcher M, Tian R, Blau JA, Markegard E, Wagner RT, Wu D, Mo X, Biton A, Zaitlen N, Fu H, McCormick F, Kampmann M, McManus MT. Nat Biotechnol. 2018 Jan 15. pii: nbt.4062. doi: 10.1038/nbt.4062. 10.1038/nbt.4062 PubMed 29334369
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