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Addgene

pISH-Pde4a
(Plasmid #105463)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105463 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-Teasy
  • Backbone size w/o insert (bp) 3015
  • Vector type
    to ganerate riboprobe

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pde4a
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    762
  • Entrez Gene
    Pde4a (a.k.a. D9Ertd60e, Dpde2)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Linearize with SpeI, use T7 RNA polymerase for IVT.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pISH-Pde4a was a gift from Peter Mombaerts (Addgene plasmid # 105463 ; http://n2t.net/addgene:105463 ; RRID:Addgene_105463)

  • For your References section:

    Trpc2-expressing sensory neurons in the mouse main olfactory epithelium of type B express the soluble guanylate cyclase Gucy1b2. Omura M, Mombaerts P. Mol Cell Neurosci. 2015 Mar;65:114-24. doi: 10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19. 10.1016/j.mcn.2015.02.012 PubMed 25701815
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