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pFA6a-GBP-RFP-kanMX6
(Plasmid #105160)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105160 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a
  • Backbone size w/o insert (bp) 4170
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    KanMX6 (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GBP-RFP-kanMX6
  • Alt name
    GFP binding protein
  • Alt name
    RFP
  • Tag / Fusion Protein
    • GBP-RFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sizes of backbone and inserts are approximate, and do not include the selectable marker.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-GBP-RFP-kanMX6 was a gift from Jian-Qiu Wu (Addgene plasmid # 105160 ; http://n2t.net/addgene:105160 ; RRID:Addgene_105160)

  • For your References section:

    Regulation of Rho-GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis. Davidson R, Laporte D, Wu JQ. Mol Biol Cell. 2015 Feb 1;26(3):453-66. doi: 10.1091/mbc.E14-07-1252. Epub 2014 Dec 3. 10.1091/mbc.E14-07-1252 PubMed 25473118
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