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pFGAMS-mEos2
(Plasmid #105128)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105128 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmEos2-N1
  • Backbone manufacturer
    Loren Looger
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PFAS
  • Alt name
    FGAMS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4014
  • GenBank ID
    NM_012393
  • Entrez Gene
    PFAS (a.k.a. FGAMS, FGAR-AT, FGARAT, GATD8, PURL)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEos2 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFGAMS-mEos2 was a gift from Stephen Benkovic (Addgene plasmid # 105128 ; http://n2t.net/addgene:105128 ; RRID:Addgene_105128)

  • For your References section:

    Spatial colocalization and functional link of purinosomes with mitochondria. French JB, Jones SA, Deng H, Pedley AM, Kim D, Chan CY, Hu H, Pugh RJ, Zhao H, Zhang Y, Huang TJ, Fang Y, Zhuang X, Benkovic SJ. Science. 2016 Feb 12;351(6274):733-7. doi: 10.1126/science.aac6054. 10.1126/science.aac6054 PubMed 26912862
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