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Addgene

pFRETgc-2in1-NN
(Plasmid #105124)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105124 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFRETgc-2in1-NN
  • Vector type
    Plant Expression
  • Selectable markers
    Basta ; lacZ
  • Tag / Fusion Protein
    • mGFP/mCherry (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • mGFP/mCherry (N terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRETgc-2in1-NN was a gift from Christopher Grefen (Addgene plasmid # 105124 ; http://n2t.net/addgene:105124 ; RRID:Addgene_105124)

  • For your References section:

    Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic Protein Interaction Studies. Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C. Plant Physiol. 2015 Jul;168(3):776-87. doi: 10.1104/pp.15.00533. Epub 2015 May 13. 10.1104/pp.15.00533 PubMed 25971551
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